Biomarker Assays

MicroConstants provides biomarker testing and analysis services to support preclinical and clinical diagnostic research of new therapeutics. We perform biomarker assay development, validation, and profiling of disease panels using multiplex, LC/MS/MS and ELISA techniques.

Multiplex Biomarker Assays (Luminex)

biomarker-assays-luminexUsing the Luminex platform for biomarker assay development, validation and profiling of disease panels enables us to simultaneously interrogate up to 100 analytes in each sample. Nucleic acids or protein samples are examined to detect biomarkers that correlate with the progression of a disease or success of a therapy.

Performing multiplex biomarker analysis using the Luminex platform with xMAP technology enables us to:

  • Profile disease panels with fast reaction kinetics
  • Develop biomarker assays with a wide dynamic range and high reproducibility, specificity, and sensitivity
  • Require smaller sample sizes than single-plex biomarker assays
  • Generate more information on interrelationships between analytes within a single sample
  • Provide the high-throughput and high-content data required for simultaneous multiple lines of investigation of complex diseases and of combinatorial correlations of coexistent pathologies

LC/MS/MS Biomarker Assays

MicroConstants develops and validates highly selective bioanalytical methods for the simultaneous quantitation of multiple biomarker peptides or epitope peptides of monoclonal antibodies in serum or plasma matrices using Solid Phase Extraction (SPE) coupled with UPLC/MS/MS. The increased sensitivity of the Xevo TQ-S triple quadrupole mass spectrometer coupled with UPLC provides the specificity, accuracy and precision required for biomarker quantitation.

ELISA Biomarker Assays

MicroConstants provides ELISA biomarker assay development and validation for biomarker identification using the following formats:

  • Competitive immunoassays for peptides and small molecules such as nucleotides and eicosanoids
  • Immunometric “sandwich” ELISA format assays for large protein analytes including cytokines, cell stress proteins, and signaling pathway regulators

Each assay undergoes rigorous testing to ensure high precision and accuracy while also delivering the sensitivity and specificity required to detect biological levels of analytes in over 35 biological matrices including blood, serum, plasma, CSF, and tears. High-throughput screening using automated instrumentation is also available.